Inhibition of Protease Produced from Clinical Isolate of pseudomonas aeruginosa using Vasicine Alkaloid

Ruaa H. Ali, Majid R. Majeed, Hiba A. Jasim

Abstract: This study was carried out using leaves of Adhatoda vasica plant, which is belongs to the family of Acanthaceae. This study includes extraction and partial purification of vacisine alkaloid and detection of active materials in the crude leaves extract using methanol 80 %. The partial purification for vasicine from crude leaves extracts was obtained by separation and precipitation with a percentage of 0.6 %. The objective of this study is determination of effect of these plant extracts as an inhibitor of proteases isolated from the pseudomonas aeruginosa bacteria, where 40 samples were collected from different hospitals in Baghdad, 25 isolates were identified as Pseudomonas aeruginosa by routine biochemical tests, Api 20 E, Then the ability of these isolates for production of proteases was screened by using quantification and semi- quantitative methods, Pseudomonas aeruginosa (P1) was selected as the highest protease producer, which was identified as one of the strains of P. aeruginosa. It was observed that The optimum culture medium and conditions for protease production by submerged cultures in the tryptic medium soya broth medium with pH 8 and incubated at a temperature of 37 C for 48 hours. The effect of vasicine on the effective protease enzyme showed that the activity of enzyme had decreased from 15.5U / ml to 1.2 U / ml when treated with vasicine at a concentration of 0.8ml. The inhibitory activity of the extract towards protease was 92.2 %.

Keywords: Protease, pseudomonas aeruginosa, vasicine, inhibitory activity