Isolation and Identification of Lipase-producing Bacteria from Oil-Contaminated Soils

Khaing Phyo Wai, Weine Nway Nway Oo

Abstract: In the present study, the indigenous microbes from oil-contaminated soil samples of oil processing mills were isolated based on their lipolytic activities. The isolated bacteria were screened on lipid-enriched media with different substrates and were selected the strains showing lipolytic activity. To be confirmed the lipase activity, the strains were further examined on olive oil and rhodamine B media. And then, the best lipase producer strains were further identified by 16S rDNA sequencing method. Based on the 16S rDNA sequencing results, the strains were close to Acinetobacter species and Stenotrophomonas species.

Keywords: oil-contaminated soil, lipase, olive oil, 16S rDNA sequencing method