Effect of Bee Venom and Other Natural Product (Dates) Onlung Cancer: Invitro Trial

Abir A. El-Fiky, Aly F. Mohamed, Eman Amin Ismail, Halima Hirzi

Abstract: Bee venom has special pharmacological activity for its enzymes and peptides containment. Melittin is the bee venoms main constituent. The present work aimed to in vitro estimation of the anti-cancer potentials of bee venom (BV) in addition to evaluation of the synergetic potential of dates extract to bee venom against lung cancer (A549). BV showed a higher toxicity toA549 cells accompanied with synergetic activity of its toxicity in combination with daters extracts where the IC50 was arranged in the order of BV> Mix> Dates compared with Doxorubicin as a positive control. Data revealed that BV/dates extract has a synergetic potential to BV that was accompanied with Anticancer activity via up/down regulation of pro and anti apoptotic genes compared with control, caspase-3, Bcl-2 andpro-apoptotic gene (p53). Cell cycle analysis revealed that bee venom, dates and their mix induced pre G1 apoptosis and cell cycle arrest at G2/M phase.

Keywords: Bee venom, dates phoenixdactylifera, A549, apoptotic, anti-apoptotic gene, pro-apoptotic gene, cell cycle, apoptosis