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Research Paper | Zoology | India | Volume 6 Issue 6, June 2017

Histological and Protein Profile Alterations of Liver of Freshwater Fish Channa punctatus (Bloch), on Exposure to Fungicide, Fytran

Deepasree.M.I ^[4] | M. S. Rajendran Nair ^[3]

Abstract: The present study was to evaluate the impact of pesticide toxicity stress symptoms in fresh water fish Channa punctatus. The effect of Fytran (Copper oxy chloride), a fungicide was assessed by exposing the experimental species Channa punctatus to sub lethal concentration of the toxicant for 24, 48, 72 and 96 hrs under static bioassay condition. From the experiments done, it was observed that at 11.82 ppm concentration of Fytran, 50 % mortality occurred. The results obtained from the calculation of LC50 showed that Fytran is toxic to the experimental species Channa punctatus. The impact of 96 hrs exposure to lethal concentration of Fytran in fresh water food fish Channa punctatus was observed. After 96 hrs of exposure, the fish tissue samples were collected from liver. The samples were analyzed for histopathological changes as well as alterations in the protein profile using SDS PAGE technique. Noticeable degenerative histopathological changes were observed in liver of Fytran treated fish samples. The protein profile also found to be varied much from the normal liver samples.

Keywords: Channa punctatus, Fytran, LC50, histopathological changes, protein profile, bioassay, SDS PAGE

Edition: Volume 6 Issue 6, June 2017,

Pages: 1370 - 1374