Caulogenesis and Chlorophyll Content in STEVIA REBAUDIANA (BERT) Bertoni by Using Glutamine in the Culture Medium

R. Thilakavathy, M. Jagadeesan

Abstract: - Incorporation of Glutamine in MS medium significantly enhanced direct shoot bud induction from nodal explants from juvenile plants of S. rebaudiana. Shoot bud induction medium was supplemented with BAP (2.5mgl-1) and IAA (2.5mgl-1) When the concentration of Glutamine in the induction medium 100mgL-1. There was significant increase in percentage response along with healthy, well developed with dark green broader leaves. Here was remarkable increase in total biomass and chlorophyll content at 100mgl-1 Level in the medium. During the proliferation stage, also presence of Glutamine in the medium favored increase in shoots bud number per explants.

Keywords: S rebaudiana, MS medium, Glutamine, chlorophyll