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Research Paper | Agricultural Engineering | India | Volume 6 Issue 1, January 2017

Characterization of ACTINOMYCETES Isolated From Rhizosphere of PISUM SATIVUM L.

Abdouslam M. Rassem | Arun A. David | Madhu B. M

Abstract: In this study Actinomycetesspp were isolated from soil samples collected from Soil Science Research Farm, SHUATS-U. P. Allahabad their provisional identification was done following the criteria of Bergey's Manual of Systematic Bacteriology. By the molecular approach the identification process was easy and can obtain results in less time. The phylogenetic analysis based on the 120 bp sequence bearing the variable and region is useful for streptomyces sp. Identification. The isolated sps subjected to 16SrRNA sequence analysis and identified as Streptomyces lucensis (AS1), Streptomyces griseus (AS2) and Streptomyces olivaceus (AS3) and biochemical and physical tests were done for all strains to identify and tolerance to conditions growth Salt, pH, and Temperature Tolerance. The strains AS, AS2 and AS3 were growth well at 28?C, 1 % NaCl and pH 6 for all strains isolated, where the strains may effect on the plant growth.

Keywords: Actinomycets, Rhizosphere, Salt, pH, Temperature Tolerance and 16S Rrna

Edition: Volume 6 Issue 1, January 2017,

Pages: 1126 - 1129