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Research Paper | Biology | India | Volume 5 Issue 11, November 2016

Analysis of Qualitative and Quantitive Constituents and Antimicrobial Activities of Aloe Vera L. against Some Selected Pathogens

Souvagyalaxmi Sahoo | Sangita Dixit

Abstract: Herbal medicines have been used in medical practices since antiquity. Aloe vera is a medicinal plant within the family Liliaceae. The present study was to investigate phytochemical residences, antimicrobial pastime of Aloe vera extract. Phytochemical screening confirmed that the methanolic extract showed best possible amount of phytochemicals compared with solvent like acetone and chloroform. Chloroform extract of roots exhibits the presence of rich phytochemicals compared to methanol and acetone. Antimicrobial activity was examined utilising disc diffusion method in three human pathogens like Bacillus subtilis, Staphylococcus aureus, and Escherichia coli and also fungus like Aspergillus niger, Fusarium oxisporum, Penicillium chrysogenum. The extraction has inhibitory effect against bacteria and fungus pathogen respectively. In our study, the TLC profiling of the different stages of Aloe vera callus, , regeneration of shoot, Aloe vera leaf and root extracts confirms the presence of a diverse staff of phytochemicals. The proposed HPTLC method presents a good resolution with gallic acid. The approach is fast, easy and specific.

Keywords: Anti microbial activity, pathogenic microorganism, TLC, HPTLC, Aloevera

Edition: Volume 5 Issue 11, November 2016,

Pages: 1438 - 1444