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Research Paper | Microbiology | India | Volume 5 Issue 11, November 2016
Antagonistic Activity of Streptomyces sp. LCC-05 Isolated from the Chennai Coast of Bay of Bengal, India
G. Laxshmi [2] | Albin T.Fleming
Abstract: Streptomyces sp. is known to produce two-third of pharmaceutically imperative antibiotics. They are predominantly found in terrestrial environment. In the current study, soil samples were collected from the Chennai coast along Bay of Bengal, India. Pre-treatment of soil was implemented by enrichment techniques paving the growth of slow growing Actinobacteria and obstructing gram negative bacteria. After serial dilution method, suspension from 10-4 dilution was inoculated in starch casein agar medium. Isolated culture inoculate from colony was examined based on morphological characteristics, that proved it to be a Streptomyces sp. and was named as Streptomyces sp. LCC-05. It was grown in Production medium-I, as it revealed favorable results and was further optimized for significant antibiotic production. Biomass free broth was mixed with equal concentration of ethyl acetate and methanol (11, v/v). Secondary metabolite extract so obtained was air dried and observed for their antimicrobial activity. They exhibited an incredible result against gram positive, gram negative and fungi. Streptomyces sp. LCC-05 like every other species from Actinobacteria possesses a tremendous antimicrobial activity and marine environment is one of the appropriate locations for isolating streptomyces sp.
Keywords: Streptomyces sp, isolation, antimicrobial activity, drug discovery and marine soil
Edition: Volume 5 Issue 11, November 2016,
Pages: 967 - 968