Expression and Relative Quantification of B29 Gene in Normal and Cancer Patients

Pooja Bhardwaj, Savita Arya, Kuljeet Singh

Abstract: B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR comprises of two transmembrane proteins Ig and Ig. Ig (CD79B) is encoded by B29 gene. Leukaemic B- cells express low to undetectable Igs, Ig (CD79B) proteins. Altered and aberrated B29 gene affect expression of Ig/CD79B protein. One of the main factor of B29 gene alteration is mutation in their coding sequence. We analyzed the quantitative expression of B29 gene in leukaemic samples. Primers for B cell expression was designed using PrimerExpress 3.0 software. Designed primers were used to analyze the expression of B29 gene in different samples through ABI prism 7000 SDS system. Relative quantification of B29 gene in samples was done by 2^-Ct method. There was under expression of B29 gene in 100 % of leukaemic samples. Though reason for lower gene expression in leukaemic samples was not detected yet there are number of papers reporting mutations and methylation as main cause of B29 gen alteration.

Keywords: B cell receptor, B29, Primer designing, Absolute quantification, 2-Ct method

How to Cite?: Pooja Bhardwaj, Savita Arya, Kuljeet Singh, "Expression and Relative Quantification of B29 Gene in Normal and Cancer Patients", Volume 5 Issue 11, November 2016, International Journal of Science and Research (IJSR), Pages: 438-442, https://www.ijsr.net/getabstract.php?paperid=ART20162764, DOI: https://dx.doi.org/10.21275/ART20162764