Determination of Aluminium with 8-Hydroxyquinoline in the Hemodialysis Waters By Liquid Chromatography of Reversed Phase Polarity

Soumaya GHARBI, Bassem JAMOUSSI

Abstract: This study highlights the benefit, as has the use of 8-Hydroxyquinoline (8-HQ), in the liquid chromatography for determination of aluminum in dialysis waters: dissolving in the mobile phase of this chelating agent allows direct injection of the aluminum without prior derivation. The retentions then depend on the one hand of the choice of the chelating agent and, on the other hand, experimental conditions related to the nature and composition of the mobile phase. We have shown that the technique by chromatography by polar reverse-phase on spectrophotometric detection in the ultraviolet (UV) enables the quantitative analysis of aluminum. The detection limit is 0.3ng for injection of 20L.

Keywords: Aluminum, 8-Hydroxyquinoline, HPLC, Hemodialysis water