Comparative Molecular Cytogenetic Analysis of Three Marine Fishes Species of Genus Epinephelus (Family: Serranidae) From Mediterranean Sea

Abu-Almaaty A. H., Hala El-Sayed Abd-Alaty, Mohamed K. Hassan, Yaseen A. Essa

Abstract: The Metaphase chromosomes of three marine fishes species of genus Epinephelus (Epinephelus aeneus; Epinephelus marginatus and Epinephelus costae (Family: Serranidae) and their karyological and molecular genetic relationships have been studied. All samples were collected from Egyptian Mediterranean Sea Coast. All three species have the same diploid chromosome number of 2n=48. Epinephelus aeneus and Epinephelus costae were identical in their karyotypes (all acrocentric) ; but the karyotype of Epinephelus marginatus was different. Nine of ten RAPD primers; showed polymorphic bands; were used for the construction of the dendrogram and a similarity matrix. A total of 51 bands were obtained; 35 of them were polymorphic. Similarity values among the studied samples ranged from 11 % to 35 %. High similarity values were obtained between Epinephelus aeneus and Epinephelus costae. (35 %) and the low similarity values were obtained between Epinephelus aeneus and Epinephelus marginatus (11 %). The cluster analysis clearly differentiated Epinephelus aeneus and Epinephelus costae from Epinephelus marginatus. RAPD analysis confirmed that the three Epinephelus species under study are genetically different from each other and a genetic variation was found between and within the three species tested in this study.

Keywords: Zoology - Cytogenetics karyotype - RAPD-PCR - DNA Fishes Serranidae Epinephelus - Mediterranean Sea Zoology - Cytogenetics karyotype - RAPD-PCR - DNA Fishes Serranidae Epinephelus - Mediterranean Sea

How to Cite?: Abu-Almaaty A. H., Hala El-Sayed Abd-Alaty, Mohamed K. Hassan, Yaseen A. Essa, "Comparative Molecular Cytogenetic Analysis of Three Marine Fishes Species of Genus Epinephelus (Family: Serranidae) From Mediterranean Sea", Volume 3 Issue 6, June 2014, International Journal of Science and Research (IJSR), Pages: 1121-1131, https://www.ijsr.net/getabstract.php?paperid=2014385, DOI: https://dx.doi.org/10.21275/2014385