Pectinase Production by Delftia Acidovorans Isolated From Fruit Waste under Submerged Fermentation

Vibha Bhardwaj, Neelam Garg

Abstract: Delftia acidovorans strain IAC/BECa-023 was isolated from waste of fruit processing unit and selected as maximum pectinase producing bacterium under alkaline conditions. The morphological; cultural and biochemical characteristics of Delftia were studied and it was identified on the basis of nucleotide homology and phylogenetic analysis. Maximal quantities of pectinase were produced in the alkaline range of the production medium at 25 C to 37 C under shaking conditions at 150 rpm in 24 hr of incubation period. Best carbon source was found to be starch supplemented with pectin and Potassium Nitrate as nitrogen.

Keywords: Delftia acidovorans, pectinase, microorganisms, optimization, submerged fermentation