Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste

Manish Kumar, Sushma

Abstract: Phytase; an acid phosphatase enzyme; efficiently cleaves phosphate moieties from phytic acid thereby generating myo-inositol derivatives and inorganic phosphate; was indentified during screening of endophytic fungi Aspergillus niger. Phytase was produced in selective media at room temperature for 3 days. Production media was also optimized varying the concentration of ingredients from 5 to 25g by using solid-state fermentation for 7 days to check the activity of phytase. The zone of inhibition of crude and pure phytase were 2.1 and 2.4cm recorded using well diffusion method.

Keywords: phytase, wheat bran waste, inorganic phosphate, Aspergillus niger, solid state fermentation