Abstract: Nanobacteria - CNPs the possibility of bacterial association of the dental calcifications in correlation with the salivary gland stones Aleksova Pavlina University Dental Clinical Center -St. Panteleimon-- Deparment of Restorative dentistry and Endodontics, Faculty of Dentistry, University -Ss. Cyril and Methodius-, Skopje, R. of Macedonia. Correspondence Aleksova Pavlina Department of Restorative dentistry and Endodontics, Faculty of Dentistry, University-St.Cyril and Methodius-, Skopje, Republic of Macedonia Tel. +389 070 260 308 E-mail firstname.lastname@example.org Introduction Nanobacteria is a cytotoxic, sterile-filterable, gramnegative, atypical bacteria that could be detected in bovine and human fluids. It can produce carbonate apatite on its cell wall. Materials and Methods The present study was analyzed dental pulp stones of the molars, and parallel operative leached salivary gland stones. Qualitative and quantitative chemical analysis was used to determine the ratio between Calcium and Phosphorus given in mg/kg and % (percentages). For the qualitative analysis of the material we used a method of Infrared Spectroscopy, known as FTIR-spectrometry. For the quantitative analysis of the material, we used a method of Atomic-absorption spectroscopy. Control group was samples hydroxyapatite. The analytical methods do not exclude the possible presence of minor quantities of other minerals. Results This analysis of dental pulp and salivary gland stones showing Ca and P peaks similar to those of hydroxyapatite. Spectrograms in absorbance of the pulp stones and the salivary gland stones were approximately equally. Mainly made up of albumin, which is the most common component of the pulp stones, and as can be seen in the spectra, of the salivary gland stones. With the method of atom-absorbing spectroscopy, we got the following results pulp stones, the value of Ca and P expressed in mg/kg was 18.501, and 11.001. The value of Ca and P expressed in % was 18.502 and 17.602. Salivary gland stones, the value of Ca and P expressed in mg/kg was 584310 and 232517. The value of Ca and P expressed in % was 58.431 and 23.252. We has presence and minor quantities of other minerals. Discussion Generally, the apatite formation required elevated Pi and/or Ca2+ levels and AP activity. In the nanobacterial model, apatite was formed at [Ca] 1.8 mM and [Pi] 0.9 mM or less. Values Ca/P ratio in dental pulp stones of this study, is such with values in the nanobacterial model. Two major chemical elements Ca and P in the sialoliths concentrations 58.43 % and 23.25 % respectively giving a Ca/P weight ratio of 2.18 which is very close to the weight ratio of pure stoichiometric hydroxyapatite 2.15. Conclusions With this study we confirm that Nanobacteria or CNPs can be the possibility of bacterial association of dental calcifications and of salivary glands stones.
Keywords: bacterial association, nanobacteria, calcifying nanoparticles, dental calcifications, salivary glands stones