Abstract: Nanobacteria can be the reason for creating sialoliths Pavlina Aleksova 1 1Department of Restorative dentistry and Endodontics, Faculty of Dentistry, University St. Cyril and Methodius, Skopje, Republic of Macedonia Correspondence Pavlina Aleksova1 Department of Restorative dentistry and Endodontics, Faculty of Dentistry, University St.Cyril and Methodius, Skopje, Republic of Macedonia Tel. +389 070 260 308 E-mail firstname.lastname@example.org Introduction Nanobacteria or calcifying nanoparticles (CNPs), i.e. propagating self-replicating nanoparticles, contribute to pathological calcification in the human body, including diseases such as dental pulp stones, salivary gland stones, kidney stones, gall stones. In this study, we identified calcium phosphate ratio, in the sialoliths or salivary gland stones. Materials and Methods The present study was analyzed operative leached 10 sialoliths. Calcium phosphate ratio, in the sialoliths, were detected in the laboratory at the Faculty of Chemistry, University, St.Cyril and Methodius in Skopje. Qualitative and quantitative chemical analysis was used to determine the ratio between Calcium and Phosphorus given in mg/kg and percentages. For the qualitative analysis of the material we used a method of Infrared Spectroscopy, known as FTIR-spectrometry. For the quantitative analysis of the material, we used a method of Atomic-absorption spectroscopy. Control group was samples hydroxyapatite. Control hydroxyapatite was correctly identified in the test. Results This analysis of sialoliths showing Ca and P peaks similar to those of hydroxyapatite. FTIR-Spectrograms in absorbance were mainly made up of albumin, which is the most common component of sialoliths, as can be seen in the spectra. The intensive line in the spectra in transmittance in the high frequency part, as well as the lines in the area around 1600 to 1300 cm-1 show the presence of albumin, and the intensive line in the area around 1000cm-1 and the doublet of lines around 600 cm-1 are the result of the vibration of phosphate ions present in the stones as carbonate apatite (Calcium Phosphate Carbonate). Atom-absorbing spectroscopy showing the value are identical of Ca expressed in mg/kg was 584310, and 58.431 in percent. The value of P in mg/kg was 232517, and 23.252 in percent. Discussion Two major chemical elements Ca and P in the sialoliths concentrations 58.43 % and 23.25 % respectively giving a Ca/P weight ratio of 2.18 which is very close to the weight ratio of pure stoichiometric hydroxyapatite 2.15. Conclusion Salivary stone formation can be nanobacterial disease initiated by bacterial infection.
Keywords: nanobacteria, calcifying nanoparticles, pathological calcification, hydroxyapatite, salivary gland stones, sialoliths