Abstract: Tulsi, a subcontinental plant has been cultivated in india for the religious and medicinal purposes. It is an erect, many branched subhurb of 30-60cm tall with hairy stem placed in lameacea family.there are aproximatly 54 compounds has been already identified in tulsi leaves, flower and spikes which are proposed to be responsible for this type of activity. E. Coli and other pathogens causing skin, oral and soft tissue infections, essential oil of tulsi could be a valuable typical antimicrobial agent for management of skin and oral infections caused by these organism. Many research and Review articles has been already published on this topic and they proposed to antimicrobial function of tulsi. The major objective of my study was to evaluate the antimicrobial activity of tulsi.oil extract, chloroform extract, and alcohol extract of tulsi has a powerful impact against different microbial pathogens like-Salmonella enteritica, Vibrio parahaemolyticus, E. Coli and Listeria monocytogenes. In general extract obtained by all method showed antimicrobial activity against all tested microorganisms. In conclusion extract of ocimum found to be containing chemical compounds useful in food preservation, development of drugs against food born and infectious microorganism.
Keywords: MIC Minimum inhibitory concentration, Essential oil components, Bactericidal, Anti fungal, mode of action