Downloading: A Simple and Efficient Protocol for Callus Induction and Regeneration from Wheat (Triticum aestivum L.) Mature Embryos
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064


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A Simple and Efficient Protocol for Callus Induction and Regeneration from Wheat (Triticum aestivum L.) Mature Embryos

Badr Eldin A. E. Saeed, Marmar A. El Siddig, Aisha Ohag Osman, Adil A. El Hussein

Abstract: aim of this study was to develop a protocol for callus induction and regeneration of 12 wheat (Triticum aestivum L.) cultivars widely grown in Sudan. Different concentrations/combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and Zeatin were tested for efficient callus, shoot and root inductions using mature embryos. Experiments were laid out in a completely randomized design with ten replicates and Duncans Multiple Range test was used for mean separation. There were significant differences between the means number of calli obtained with different 2, 4-D concentrations tested. The highest frequencies of induced embryogenic calli were recorded in Murashige and Skoog (MS) medium containing 2.0 mg/l of 2, 4-D. Different wheat cultivars displayed variable frequencies in their response to each of the six 2, 4-D concentrations indicating genetic variability and that wheat regeneration is genotype dependent. Shoots and roots were successfully induced in MS medium supplemented with 2.0 mg/l 2, 4-D and 1.0 mg/l Zeatin. The highest regeneration capacities of more than 40 % were recorded for cultivars Sasareeb (49 %), Nebta (45 %) and Imam (42 %), while the lowest (19 %) was recorded for cv. Nasser. This simple and reproducible regeneration protocol can be used in any future biotechnological program for wheat improvement in Sudan.

Keywords: Wheat, callus induction, embryogenesis, regeneration


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