Downloads: 111
Research Paper | Botany | India | Volume 4 Issue 6, June 2015
Rapid in Vitro Plant Regeneration from Nodal Explants of Withania somnifera (L.) Dunal: a Valuable Medicinal Plant
Syed Saema | Iffat Zareen Ahmad | Pratibha Misra [2]
Abstract: Withania somnifera (L. ) Dunal is a high demanding valuable medicinal plant which have pharmaceuticals properties, known for its withanolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog-s, 1969) medium horizontally containing different concentration (1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axillary ends of explants when kept horizontally in the medium, within 3 weeks of culture incubation. Maximum multiple shoots was found in MS medium supplemented with (2.0mg/l) BAP as it induced an average of 21 0.0.57 shoots per explants and the maximum shoot length (5.24 0.20) cm. Successful in vitro rooting was induced from cut end of the microshoots when placed on half-strength MS medium supplemented with (5.0mg/l) Indole-3-butyric acid (IBA). The regenerated shoots with well developed root system were successfully acclimatized and established in pots containing soilleaf manure (31) and grown under greenhouse conditions with 94 % survival rate.
Keywords: Withania somnifera, BAP, nodal explants, in vitro tissue culture, plant regeneration
Edition: Volume 4 Issue 6, June 2015,
Pages: 1649 - 1652
Similar Articles with Keyword 'Withania somnifera'
Downloads: 3 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2
Research Paper, Botany, India, Volume 13 Issue 2, February 2024
Pages: 431 - 434Comprehensive Exploration of Withania somnifera (Ashwagandha): A Quantitative Analysis of Bioactive Compounds and Traditional Applications
D. P. Chavan
Downloads: 120
Research Paper, Botany, India, Volume 5 Issue 2, February 2016
Pages: 2209 - 2214Enhancement in Growth and Yield of Ashwagandha (Withania Somnifera (L.) Dunal) by Counteracting Effect of Infrared Supplemented with Ultraviolet-B Radiation
Manju Nagar | Surchi Tyagi [2] | Ashwani Kumar Goyal [2]