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Research Paper | Microbiology | India | Volume 4 Issue 5, May 2015

Antibiogram of Various Bacterial Isolates from Pus Samples in a Tertiary Care Centre in Rajasthan

Dr. Swati Duggal | Dr. P K Khatri | Dr. R S Parihar | Dr. Rajat Arora

Abstract: Introduction- Pus is an important clinical specimen received in Microbiology laboratory for aerobic culture and sensitivity. The pace of development of drug resistance in bacterial isolates far exceeds the rate of newer drug discoveries highlighting the importance of conducting periodic studies to determine their antibiogram. Materials and Methods- Pus samples from different departments of Mathuradas Mathur Hospital were collected using aseptic technique for 3 months duration and were processed in the laboratory immediately using standard microbiological procedures. Identification of bacteria was carried out with motility testing, Gram staining and biochemical reactions. The antibiotic sensitivity testing of all isolates was performed by Kirby Bauer-s disc diffusion method on Muller Hinton agar and interpreted as per CLSI guidelines. Results-Analysis of 119 pus samples showed 93.27 % culture positivity (MF=1.481.00) with Surgical wards (32.43 %) being the major contributor. Pseudomonas (29.73 %) was the most common organism followed by Staphylococcus aureus (22.52 %). Gram positive cocci were susceptible to Linezolid (94.87 %), Vancomycin (92.31 %) and Imipenem (92.31 %) whereas Gram negative bacilli were susceptible to Imipenem (87.10 %), Piperacillin (61.29 %) and Gentamicin (48.39 %). Conclusion-The changing trends of antimicrobial susceptibility in bacterial isolates from pus can serve as a useful tool for physicians to start empirical treatment of patients at the earliest according to the geographical areas and emerging multi-resistant bacteria.

Keywords: Antibiogram, Imipenem, Pseudomonas, Pus, Staphylococcus aureus

Edition: Volume 4 Issue 5, May 2015,

Pages: 1580 - 1584

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