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Research Paper | Botany | India | Volume 4 Issue 5, May 2015

Phytochemical Analysis of Antioxidants From Abutilon indicum L. and Paederiafoetida L. Supported by TLC, FTIR and NMR Studies

Rajalaxmi Nayak | Ashok Panda | Luna Samanta | Santilata Sahoo

Abstract: Abutilon indicum and Paederia foetida are two important medicinal plants in traditional healing. Abutilon indicum has proved antibacterial, analgesic, antimalarial property. Studies also show that it has hepato-protective and wound healing capacity. Similarly Paederia foetida, a perennial twinner belonging to family Rubiaceae used for gastric disorder and rheumatic condition. It has also hepato-protective and antidiabetic properties. In present study phytochemical screening of both the plants were done with help of common detection test for antioxidants generally found in plant system. Phytochemical fingerprinting was done with TLC studies where methanolic plant extract were loaded along with standard antioxidant compounds. The result of above studies was backed up by UV, FTIR and NMR studies. The screening confirmed that both the plants have secondary metabolites contributing to their antioxidant potentiality. The DPPH scavenging assay shows lower IC50 for Abutilon indicum i. e.52g/ml than 98g/ml in case of Paederia foetida. Estimation of total flavonoid content shows Abutilon indicum was having higher phenolics and lower flavonoid content whereas Paederia foetida has almost equal phenolic with high flavonoid content.

Keywords: Phytochemical screening, TLC fingerprinting, DPPH assay, Total phenolic and flavonoid, FTIR, NMR, UV

Edition: Volume 4 Issue 5, May 2015,

Pages: 1312 - 1318

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