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Research Paper | Biology | India | Volume 4 Issue 2, February 2015

In vitro Biofilm Formation of Pseudomonas fluorescens, A Promising Technique for Waste Water Treatment

Ravishankar Naik | Vaishali Pawar | Deepika Suryawanshi

Abstract: In recent, the search of plant growth promoting rhizobacteria (PGPR) for sustainable crop production is increasing drastically. The Pseudomonas fluorescens, isolated from rhizosphere of wheat fields analyzed for bio-control agents, PGPR, biofilm formation for waste water treatment. The isolates showing fluorescence under UV light, characterized using morphological and biochemical characteristics. Pot assay proved its growth promoting ability by enhancing growth of Vigna radiata plants (treated) compared to control (untreated) and antibacterial and antifungal activity was evaluated against rhizospheric bacteria (Rhizobium leguminosarum and Azotobacter agilis) and fungal pathogens (Aspergillus niger and Fusarium oxysporum) respectively. No inhibition seen against rhizospheric bacteria but inhibit fungal pathogens indicating quorum sensing mechanism involved in biocontrol activity. The efficiency in vitro biofilm formation and effect of different concentrations of antibiotics on biofilm formation was studied. This biofilm used to treat municipal waste water by passing waste water through it by maintaining constant flow rate of 1ml/min. Effective use of biofilm formed for enhanced waste removal in wastewater treatment application was studied with pH, temperature, color, determination of solid waste and MPN test. Results from this study provide application of this bacterium as PGPR, control fungal root diseases and for purification of waste water increasing its commercial use.

Keywords: Pseudomonas fluorescens, Biocontrol agents, Pot assay, Biofilm formation, Waste water treatment

Edition: Volume 4 Issue 2, February 2015,

Pages: 1602 - 1606

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