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Research Paper | Agronomy | Philippines | Volume 4 Issue 2, February 2015

Isolation and Characterization of Indigenous Microorganism (Imo) from Ifugao Bamboo (Phyllostachys Aurea) Forest

Chiemela F. Anyanwu ^[3] | Serafin L. Ngohayon ^[3] | Ricardo L. Ildefonso ^[2] | Joseph L. Ngohayon ^[2]

Abstract: This investigation was conducted to isolate and characterized samples of indigenous microorganism (IMO) collected from bamboo forest in Ifugao province Philippines. Plate count was used to determine the relative proportion of different types of microbes in the sample, either as bacteria, fungi or yeast. The samples were serially diluted and the dilutions (10-1 - 10-8) were plated on selective media. Microbial isolates showed high bacterial population compared to normal soil population. Eight (8) bacterial isolates as pure culture were relatively the dominant microbes with population ranging from (1.4 x 109 to 5.5 x 108 CFU/g). Bacterial and fungal isolates exhibited distinctive cultural characteristics (color, shape, colony size (mm), texture, elevation, appearance, optical property). Based on the findings of this present investigation, the authors concludes that useful potential bacteria and fungi as indigenous microorganism can be collected from bamboo forest in Alfonso Lista, Ifugao Philippines which can be isolated and used for further studies on bioconversion of farm waste into organic fertilizer.

Keywords: Microorganism, IMO, bamboo, cultural characteristics, culture media

Edition: Volume 4 Issue 2, February 2015,

Pages: 1319 - 1324

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