Ihsan Edan Alsaimary, Falih Hmood Mezban
Abstract: This study aimed to detect IL 4 and IL 17 by conventional and RT PCR. DNA-based techniques have been further simplified benefiting from the introduction of PCR the result of this estimation revealed that the amplified DNA (PCR product) was 710 bp for IL-4 (50 voltage for 1 hour) and the presence of IL-4 was 85% from total samples, (PCR product) was 344 bp for IL-17 on agarose gel (1%) The presence of IL-17 was 70 % from total samples. RT PCR IL-4 show peak between 84.5 ? 85.2 and IL-17 peak between 86.5-87.5.
Keywords: conventional and Real Time q-PCR techniques, human bronchial asthma, interleukins