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Research Paper | Microbiology | Nigeria | Volume 9 Issue 5, May 2020

Antibacterial Activities of Fungi Isolated from Soil Samples in Refuse Dumping Sites at IFITE-AWKA in AWKA South Local Government Area of Anambra State

Odumodu Obinna Albert | Okpalla Jude

Abstract: In the study of antibacterial activities of fungi isolated from soil samples in refuse dumping sites at Ifite-Awka in Awka south local government area of Anambra state, one sample each was collected from 5 different sites A-E and analyzed for fungi isolations using conventional methods. A total number of 9 fungal specie were isolated which includes Aspergillus niger, Mucor microsporus, Candida albicans, Aspergillus fumigatus, Rhizopus stolonifer, Rhizopus microsporus, Penicillium janthinalium, Mucor indicus and Alternaria alternata were isolated and screened for antibacterialactivities against 4 different test bacterial which includes; Bacillus subtilis, E. coli, Salmonellaenterica serovarTyphi and Staphylococcus aureus clinically isolated from urine, stool, mouth and sour swab samples of patients in the intensive care unit of Nnamdi Azikiwe universityteaching hospital Nnewi in Anambra State. The bacterial isolates were subjected to biochemical confirmatory test before use. Thecrude extracts of isolates with highest zone of inhibition in all the sampling sites showed that in site A, E. coli shown clear zone of inhibition of 18mm for Mucor indicus while in site B, Staphylococcus aureus, E. coli and Salmonella enterica serovar Typhi showed 5mm, 10mm and 13mm respectively for Aspergillus niger. In site C, Salmonella enterica serovar Typhi showed the lowest zone of inhibition of 4mm for Aspergillus niger while the highest zone of inhibition of 19mm was shown in site D by S. enterica serovar Typhi for Aspergillus niger. Site E showed 16mm clear zone of inhibition as the highest zone of inhibition respectively. The minimum inhibition concentration (MIC) and minimum bacteriocidic concentration (MBC) for S. enterica serovar Typhi showed negative growth while E. coli, Staphylococcus aureusand Bacillus subtilis showed positive growth against Aspergillus niger respectively at the lowest concentration of 1.6mg/ml. Salmonella enterica serovarTyphi had the highest clear zones of inhibition for Aspergillus niger in all the sampling sites indicating that it produced bioactive antibacterial compounds that inhibits bacterial growth followed by E. colifor Mucor indicus while Salmonella enterica serovar Typhi also showed the lowest zone of inhibition for Rhizopus microsporus.

Keywords: Antibacterial, Pathogenic, Metabolites, Bioactive compounds, Minimum inhibition concentration, Minimum bactericidal concentration, Antagonism, Refuse dumping sites

Edition: Volume 9 Issue 5, May 2020,

Pages: 1107 - 1112

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