Dr. Brij Mohan Singh
Abstract: The study has been investigated that liver responsible for the detoxification of damaging electrophiles generated during oxidative stress in mammals. Although liver is enriched with endogenous antioxidants, but exogenous antioxidants are more helpful to detoxify toxicity generated by prooxidants. Author found decreased LDL activity in cyclophosphamide (CP) administered mice (90.36 %) and further increase in flaxseed supplementation group (131.51 %) indicating the protective mechanism of lignan component of the flaxseed oil. Similarly, significant increase were found in HDL, Cholesterol, triglycerides activities after CP administration were increased by 104.07 %, 108.84 % and 185.78 % in comparison to control group (32.90 ± 0.34, 14.70 ± 0.41 and 12.80 ± 0.80 µg/ml) and further increased in flaxseed treated mice by 140.18 %, 155.78 % and 400.46 % respectively in comparison to control group of mice. There was also a remarkable elevation in the serum protein markers TNF-α, ALP, ALT, and AST. They were increased by 202.10 %, 254.95 %, 604.08 % and 172.75 % in CP administered group in comparison to control group and further increased by 241.68 %, 492.48 %, 366.78 % and 142.29 % in flaxseed supplemented group of mice. These pathological and biochemical changes correlated well with the altered enzyme activities. The results indicate the anti-oxidative properties of flaxseed oil which showed its prophylactic property against the cyclophosphamide-induced biochemical alterations in mice liver.
Keywords: Cyclophosphamide, prooxidants, oxidative stress, flaxseed oil