Downloading: Purification and Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Telangana Region
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



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Purification and Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Telangana Region

T R Satyakeerthy, T R Shanthi, Raju Bolla, Lini Nirmala

Abstract: Polyphenol oxidase (PPO) from Zyzyphus spina-christi from Telangana region was extracted and purified by (NH4)2SO4 precipitation, ion-exchange chromatography and gel filtration chromatography. The biochemical characteristics of reveals that this PPO has higher affinity towards catechol (Km = 12.2mM and Vmax = 17, 200 U/ml min-1) at an optimum pH of 5.75. The enzyme has an optimum temperature of 37.50C and was relatively stable up to 480C for a period of 60 minutes with almost 78% of activity remaining. Among the various polyphenol oxidase inhibitors tested, the most effective inhibitor for the enzyme with 10mM catechol as substrate was ascorbic acid.

Keywords: Polyphenol Oxidase, Zyzyphus spina-christi, Telangana, Catechol, inhibitor, Optimum



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