Dhanya T, Anjana T S
Abstract: Benzimidazole and its amine derivatives having promising activities like anticancer, antibacterial, antiparasitic, antihistamine, antiulcerative, antineoplastic, antifungal, antitubercular, anti HIV etc. In this view of these observation it was thought of interest to undertake the design and synthesis of compounds having Benzimidazole moiety and evaluate their anticancer activity. To select and prepare targets of interest and carry out the docking studies of analogues using Schrodinger software to obtained the docking scores, Five different Benzimidazole analogues were synthesized by both conventional and microwave menthod, the purity of the compounds was checked by TLC, the structure of compounds were confirmed by IR, NMR, UV and Mass spectra. preliminary pharmacological screening of the syntheisized analogues were performed, which showed promising glide score was screened for anticancer activity against HEPG2 cell line.
Keywords: MTT Assay, HEPG2 Cell, Schrodinger, JAK/STAT Pathway, 5-Fluorouracil