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Research Paper | Molecular Engineering | India | Volume 3 Issue 9, September 2014

Genomic DNA Isolation from Fungi, Algae, Plant, Bacteria and Human Blood using CTAB

Mritunjay Singh Kumar | Gurneet Kaur | Avneet Kaur Sandhu

Abstract: DNA isolation with good intensity and quality is always been a necessity, whether you need for PCR or any other analysis. Cetyltrimethylammonium bromide (CTAB) buffer is used for DNA extraction from plants with various other extraction buffers. CTAB is mainly used in extraction of DNA from plants species. Here we have used CTAB for DNA extraction not only from plants but various other samples like FUNGI, ALGAE, BACTERIA, and HUMAN BLOOD. In this paper, not only isolation is performed from various samples but also an optimized protocol is introduced. Same protocol is used for all the samples for extraction only the concentration used varies. This method is basically introduced for Universities where there is time limit, Research centres where DNA extraction has to be quick enough with good quality and intensity in just one go and also can be used in High schools where students can easily handle with no risk of injury and extraction can be done quickly. This method is cheap enough to handle expenses in any organization as well. We have not used any other extraction buffer. CTAB buffer with beta-mercaptoethanol is used that helps in degradation of polysaccharides and proteins to get pure form of DNA.

Keywords: DNA extraction, CTAB, DNA isolation from CTAB, Cetyltrimethylammonium bromide, Genomic DNA

Edition: Volume 3 Issue 9, September 2014,

Pages: 617 - 618

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