International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 135

Research Paper | Microbiology | India | Volume 5 Issue 6, June 2016


In-vitro Transit Tolerance of Probiotic Bacillus species in Human Gastrointestinal Tract

Ajitha S. Nair | Aparna V. Dubhashi


Abstract: The present work describes the tolerance of five potential probiotic Bacillus species towards acid and bile in human gastrointestinal tract by in-vitro studies. Artificial gastric and intestinal juice was prepared and adjusted to different pH values for acid and bile tolerance study. A time course analysis up to 4 hours was studied with respect to viability. There was a statistically significant growth response of isolates under high acidic conditions (p < 0.05) as confirmed by ANOVA test. Bacillus VS-5 was found to be the best with 98.78 % survival rate for 3 hours exposure to pH 1.2 and 98.10 % to 2 % bile concentration when exposed for 4 hours. Considering the residence time of food in stomach and intestine, this isolate proves to have an advantage of surviving and also proliferating in the intestinal tract, which is a desirable characteristic of probiotic cultures.


Keywords: Probiotic, Bacillus species, transit tolerance, gastric juice, ANOVA test


Edition: Volume 5 Issue 6, June 2016,


Pages: 1899 - 1902


How to Download this Article?

You Need to Register Your Email Address Before You Can Download the Article PDF


How to Cite this Article?

Ajitha S. Nair, Aparna V. Dubhashi, "In-vitro Transit Tolerance of Probiotic Bacillus species in Human Gastrointestinal Tract", International Journal of Science and Research (IJSR), Volume 5 Issue 6, June 2016, pp. 1899-1902, https://www.ijsr.net/get_abstract.php?paper_id=NOV164343

Similar Articles with Keyword 'Probiotic'

Downloads: 42

Review Papers, Microbiology, India, Volume 10 Issue 3, March 2021

Pages: 954 - 957

Microbial Contamination of Yoghurt-An Overview

S. Kisanthini | M. B. Kavitha

Share this Article

Downloads: 102

Research Paper, Microbiology, Iraq, Volume 4 Issue 4, April 2015

Pages: 2990 - 2992

The Antagonism of Enterococcus Fecalis Isolated from Kefir with Salmonella Typhimurium Caused Poultry Diarrhea in Vitro

Alshawii | Adian Abdul AL-Razaq Dakhel Majid Amel

Share this Article
Top