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Research Paper | Chemistry | Malaysia | Volume 5 Issue 4, April 2016

Phytochemicals Screening and Antioxidant Activities of Aqueos Leaves Extract of Euodia ridleyi and Colubrina asiatica

Nik Hairiah Nik Mohamad Ravi | Nor Azila Maskam ^[2] | Husna Hawa Mohd Hassan | Maryana Mohamad Nor

Abstract: This study was conducted to identify the presence of phenol, flavonoids and antioxidants resulted from the aqueous extracts of the leaves of Euodia ridleyi (Tenggek burung) and Colubrina asiatica (Peria pantai). The established conventional methods were used for quantify the total phenol, flavonoids contents and antioxidant activity. Phytochemicals screening were conducted to determine the presence of flavonoids, tannin, saponin, terpenoid and phenolic content whilst DPPH method were used in determining total antioxidant activities. The positive result obtained from a screening test for both plants as it showed the presence of flavonoids, tannin, saponin, terpenoid and phenolic content. The analysis of total phenolic showed E. ridleyi contains 44.56 0.35 mgGAE/g and 20.97 0.53 mgGAE/g for C. asiatica. The result of total flavonoid showed C. asiatica has contained 1.510.09 mgQE/g and 0.140.03 mgQE/g for E. ridleyi. Concentrations of the plant extracts required for 50 % inhibition of DPPH radical scavenging effect (IC50) were recorded as 5.04 2.17 for E. ridleyi and 5.85 0.17 for C. asiatica. Thus, this study is useful in developing plant based antioxidant, which expected to be superior to synthetic antioxidant especially in the development of drugs to treat various diseases.

Keywords: Euodia ridleyi, Colubrina asiatica, phytochemicals screening, antioxidant activity, DPPH assay

Edition: Volume 5 Issue 4, April 2016,

Pages: 2004 - 2007

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