International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 127

Research Paper | Biology | India | Volume 5 Issue 4, April 2016


Characterization of Bacteria Found in Soft-Drink through Biochemical Analysis and 16s rDNA PCR

Ashutosh Kumar Mishra [2] | Pushpasingh | Himani Chugh | Brijesh Lohchania | Monica Srivastava


Abstract: Western culture brings us soft drinks as a part of our daily meal. It is aerated beverages which is very easily replacing water from our drinking habits. Ingredients of soft drinks help bacteria to survive in very odd conditions also. Here, efforts are being made to identify if any bacteria is found in our collected samples from open market. To characterize bacteria found we used various techniques like biochemical tests and 16s PCR. We used universal primer to complete PCR. Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities.16sr PCR is a technique to identify conserved sequence of bacteria.16sr PCR gel electrophoresis image is analyzed using TotalLab Quant version 12.4 software. This software analyses gel image with respect to ladder run along with samples and generates reports in term of molecular weight of each electrophoresis band.


Keywords: 16s rDNA, Biochemical test, Total Lab Quant, Soft drink, Universal primer


Edition: Volume 5 Issue 4, April 2016,


Pages: 321 - 324


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How to Cite this Article?

Ashutosh Kumar Mishra, Pushpasingh, Himani Chugh, Brijesh Lohchania, Monica Srivastava, "Characterization of Bacteria Found in Soft-Drink through Biochemical Analysis and 16s rDNA PCR", International Journal of Science and Research (IJSR), Volume 5 Issue 4, April 2016, pp. 321-324, https://www.ijsr.net/get_abstract.php?paper_id=NOV162564

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