Dr. Dande Swapna Sree
Abstract: Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution method and the microorganisms isolated were screened for antagonistic effect against Aspergillus flavus. Two fungal strains namely Penicillium sp, and Trichoderma asperellum and four bacterial strains namely Pseudomonas fluorescence, Bacillus megaterium, Bacillus subtilis and Bacillus sp. were isolated and identified through morphological and biochemical characteristics from rhizosphere of groundnut. Of the above Trichoderma asperellum among fungal isolate and Pseudomonas fluorescence from bacterial isolate showed more antagonistic effect against Aspergillus flavus and thus proved to be potent biocontrol agents. Two talc-based formulations one from Trichoderma asperellum and another from Pseudomonas fluorescence were developed as an integrated disease management package against Yellow mold caused by Aspergillus flavus in Groundnut (Arachis hypogea L.). Trichoderma asperellum was multiplied in vitro in molasses-yeast medium and the biomass along with the medium was incorporated into the talc. The talc was packed and stored in polythene bags. Similarly Pseudomonas fluorescence was multiplied in nutrient broth and formulated into talc based product. This was packed and stored in polythene bags.
Keywords: Groundnut, Aspergillus flavus, Yellow mold, Antagonism, Integrated disease management