M.G.A.EL.Sayed, Enas. A. H. Farag, Heba. M. Nasr
Abstract: Exposure to carbon tetrachloride induces acute and chronic hepatic injuries as well as renal injuries in rats. Therefore, the current study aimed to evaluate the effect of probiotic (Lactobacillus acidophilus) on hematology, blood biochemistry and lipid profile of healthy rat, also the protective role against carbon tetrachloride -induced toxicity in albino rat. Four groups with ten rats each group were used for this purpose; these groups included the control vehicle group that received saline daily for 30 days, probiotic group (0.009x106 CFU/ gram of rat body weight orally) daily for 30 days; carbon tetrachloride group (2.5ml/kg intraperitoneally twice per week for three week; the probiotic carbon tetrachloride group. The results revealed that carbon tetrachloride significantly increased serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, cholesterol, triglyceride, urea and creatinine. In addition, there were substantial increase in lipid peroxidation (malondialdehyde), white blood cell, packed cell volume and level of glucose with significant decreases in albumin, total protein, creatinine kinase, hemoglobin and red blood cells. Carbon tetrachloride also caused histological changes in liver, kidney and spleen tissues. However, administration of probiotic ameliorated the carbon tetrachloride induced liver and kidney damage with improved hematological, lipid profile and glucose level.
Keywords: Probiotic, Lactobacillus acidophilus, hematology, lipid profile, blood biochemistry, carbon tetracholoride