Albehwar A.M., El-Dakhly A.T, Mona A.M. El-Manzalawy, Ekbal, M. Farouk, Heba, M. Ghanem
Abstract: The authors have been succeeded through this work to prepare horse anti-canine distemper (CD) and anti- canine parvo (CP) hyper immune serum as a local product provided on time of request instead of the imported one which is usually unavailable and of high cost. Such antiserum was found to be free from foreign contaminants and safe inducing no abnormal local or systemic signs in inoculated animals. It was found that this antiserum had CD and CP antibody titers 512 and 1024 respectively as titrated by the Antibody Quantitate ELISA kits. These levels of antibodies were able to overcome CD in naturally infected and contact infected puppies in a percentage of 33.3 and 100 % respectively. While in case of CP naturally infected and in contact infected puppies this ratio was 100 % so it could be said that the prepared horse anti-CD and anti-CP hyper immune serum could be used as emergency management of CD and CP diseased puppies lowering disease progress and economic losses caused by death of these animals.This work indicated also that the Chromatographic Immunoassay as a rapid assay for detection of canine distemper (CD) and canine parvo (CP) virus antigen was less sensitive than other serological tests giving positive results of 57.1 and 54.4 % of examined samples to CD and CP antigens respectively confirming the recorded disease signs on naturally infected puppies.
Keywords: canine distemper, canine parvo, hyper immune serum