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Research Paper | Pharmacology and Toxicology | Indonesia | Volume 9 Issue 5, May 2020

Effectiveness of Combination Concentration of Lime Leaves Extract and Galangal Rhizome on Mortality of Aedes Aegypti Larvae

Yohanes Mau Abanit | Muntasir ^[7] | Pius Weraman ^[4]

Abstract: Dengue Hemorrhagic Fever (DHF) is a contagious disease and often appears to be Extraordinary Event as it spreads very fast and can cause death. Efforts to prevent DHF can be done by breaking up the life cycle of chemical vectors by utilizing lime leaves (citrus aurantifolia) and galangal rhizomes (Alpinagalanga) which have the potential as natural larvicides because they contain chemical compounds consisting of limonoids, flavonoids and phenols. The City of Kupang is a yearly endemic area of dengue where the case of the disease has increased in the last three years. This study is aimed at determining the effectiveness of the combination of the concentration of lime leaf extract and galangal rhizome on the mortality of Aedesaegypti larvae. This type of research is a pure experiment using a completely Randomized Design as the research design. There were three treatments of concentration used, namely 0 % concentration, LC50 combination of lime leaf extract and galangal rhizome, LC90 combination of lime leaf extract and galangal rhizome with a number of repetitions three times. The results of the study, the combination of LC50 of lime leaf extract and galangal rhizome was able to cut off the life cycle of 19 larvae (76 %), while the combination of LC90 of lime leaf extract and galangal rhizome was able to completely cut off the larval life cycle of 25 larvae (100 %). The result of the One-Way Anova Test analysis on the treatment of a combination of LC50 and LC90extracts of lime leaves and galangal rhizomes to Aedes Aegypti larvae mortality during 24 hours obtained sig (p) = 0.00 (p <0.05) showed that the combination of LC50 extract lime leaves and galangal rhizome and LC90combination of lime leaf extract and galangal rhizome have the effect of treatment on the number of larvae deaths of Aedesaegypti instar III and IV. The Least Significant Difference Test (LSD) analysis of the three treatment concentrations of Aedesaegypti larvae mortality showed a difference in treatment because a sig (p) = 0.00 (p <0.05) was obtained.

Keywords: Combination of Concentration of lime LeafExtract, Combination of Galangal Rhizome Extract Concentration, LC50, LC90

Edition: Volume 9 Issue 5, May 2020,

Pages: 492 - 494

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