Downloading: Coir Pith as a Acclimatizing Medium for Micropropagated Capsicum SP
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064

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Coir Pith as a Acclimatizing Medium for Micropropagated Capsicum SP

Immanuel Suresh J, Samuel Paul Raj

Abstract: Micropropagation system was developed through an enriched culture system for Capsicum annum L. of a traditional medicinal plant and nutritionally important spice crop. Proliferation of shoots were achieved on MS medium supplemented with 13 g l-1 sucrose, 8 g l-1 agar and fortified with benzyladenine (BAP) in the rage of 0.2 -1mg/l. A higher number of shoots were observed on an enriched MS medium supplemented with 0.5mg/l BAP. Then the shoots were subjected for root induction with the concentration of 1mg/ml. For the multiple shoot induction different combination of hormones were used. The well-developed microshoots were transferred to rooting medium supplemented with IAA (1.0mg/l) for efficient rooting. The well developed plants were transferred for hardening with coir pith, because of its water holding capacity, C: N ration, pH and the porosity. The efficiency was achieved around 90 % survival of the micropropagated plants.

Keywords: Micropropagation, coir pith, shoot



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