Hassanain Hataf Jaber, K. Rajyalakshmi, M. Kishore Babu, A. Krishna Satya
Abstract: In the present study Nearly Threatened Woody Tree Species Pterospermum Xylocarpum was selected for the screening of crude extracts [Hexane, Chloroform, Methanol and Aqueous (Water) extracts]for their anti-diabetic activity both in vitro and in vivo. In vitro anti-diabetic activity was done by both Alpha-Amylase inhibition assay and by Glucose uptake in Yeast Cells assay.The in-vitro analysis reveals that the Methanol extract inhibits the amylase enzyme along with the standard drug. In Vivostudies Male Sprague Dawley (150 180gms) rats were used. The animals were maintained under standard environmental conditions and were fed with standard pellet diet and water ad libitum. The experimental protocol was approved by the Institutional animal ethics committee. CPCSEA guidelines were adhered during the maintenance and experiment. Four extracts [Hexane, Chloroform, Methanol and Aqueous (Water) ] have been tested for the acute hypoglycaemic activity and there was a significant change in the methanol extract administered rat models.
Keywords: Anti-diabetic activity, in-vitro, in- vivo studies, Methanol extract