Dr. Chetan Chavan, Chanakya Thaker, Chetan Chaudhari
Abstract: An accurate, simple, reproducible, and sensitive method for the estimation of Iron (III) and Iron (II) was developed and validated. Iron (III) and Iron (II) weres separated using chelation ion chromatography technique using isocratic elution with a flow rate of 1.2mL/min. Post column derivatization with 4- (2-pyridylazo) resorcinol (PAR) was carried out to form light abosrbing complex an detected at 530nm.The mobile phase include pyridine-2, 6-dicarboxylate (PDCA) which acts as chelating agent and helps to retain Iron (III) and Iron (II) on mixed mode separator column. The linearlity of method has been tested in the range of 5.0mg/L to 15mg/L of Iron (III) and 0.5mg/L to 1.5mg/L of Iron (II) and correlation cofficient (R2) was >0.999 for both Iron (III) and Iron (II). The method was shown excellent reproducible, linear, specific, sensitivity, rugged. The Limits of Detection and Quantification have been also established for Iron (III) as 0.05mg/L & 0.1mg/L and for Iron (II) as 0.3mg/L & 0.5mg/L respectively. Hence, the validated method is easy to adapt for regular analysis.
Keywords: Iron Sucrose, Iron Polymaltose, Post Column Reagent, Iron Speciation, Ion Chromatography, Chelation, PDCA, PAR