Bahaa Noor Madhloom, Ameena Ryhan Diajil
Abstract: Background hypertension is a condition in which the blood vessels have persistently raised pressure, putting them under increased stress. Oxidative stress is an imbalance between ROS and antioxidant defense mechanisms, causing damage to biological macromolecules and dysregulation of normal metabolism and physiology. Oxidative stress is contributes to the etiology of hypertension in humans. Capoten is an angiotensin-converting enzyme (ACE) inhibitor. Which is responsible for the conversion of angiotensin I to angiotensin II. The aim of this study was to assess the oxidative stress in hypertensive patients on Capoten treatment through the assessment of salivary Malondialdehyde (MDA) and superoxide dismutase (SOD) as a marker of oxidative stress. Material and method 60 individuals were included in this study, divided into two groups, one study group and one control group. The first group composed of 30 hypertensive patients on Capoten antihypertensive agent. The second group (control group) composed of 30 healthy subjects without any systemic disorder and almost healthy oral hygiene. Intraoral examination was done for each individual. Saliva samples were collected in restful and quit circumstances, the salivary flow rate (F/R) was calculated ml per minute. PH of salivary secretion were measured by PH meter. The levels of salivary MDA and SOD were analyzed by using ELISA kit based on the principle of Competitive enzyme immunoassay technique, the concentrations of markers were measured by spectrophotometer at 450nm in a microplate reader. Results salivary MDA was significantly higher in patients groups in relation to control group. Salivary SOD was significantly lower in patient groups in relation to control group. Salivary flow rate and PH was significantly lower in patient groups comparing to control group. Conclusions salivary MDA and SOD can be used as potential marker for monitoring patients with Hypertension.
Keywords: Oxidative stress, hypertension, Capoten, MDA, SOD, Salivary flow rate and PH