Najlaa Nabhan Yaseen, Fatima Hassan Ali
Abstract: A total of twenty isolates of Pseudomonas aeruginosa were gained after their diagnosis biochemically, APi 20 system and PCR technique. These isolates were isolated from patients with different infections. They were tested to detect their ability to form biofilm, and the strongest one was chosen (named N20) to complete this study which aimed to study the effect of pomegranate (Punica granatum) peel aqueous and alcoholic extracts in addition to Imipeneme antibiotic on biofilm, swarming motility and pyocyanin production. Results showed high decrease in biofilm values by using these extracts especially in case of biofilm formation rather than degradation. MIC values were detected in order to determine sub-MIC values that were used in this study. MIC was 25 mg/ml for aqueous extract, while alcoholic extract was more effective and it', s MIC was 12.5mg/ml. Imipeneme MIC was 64g/ml which is considered very high value and indicate N20 resistance to it. Both swarming motility and pyocyanin production were inhibited by addition of these extracts and antibiotic separately to swarming media and Nutrient agar respectively when comared with control plates.
Keywords: Pseudomonas aeruginosa, Biofilm, Swarming motility, Pyocyanin