Mohammad K. Hammood, Maryam A. Khalaf
Abstract: In this article, Chloramphenicol (CHP) was determined via local made spectrofluorometric using solid state laser diode 405 nm as a source combined with flow injection technique. The proposed method is characterized by sensitive, accurate and speed for the determination of CHP in different pharmaceutical formulations. The procedure depends on the fluorescence of 2, 7-dichlorofluorescein as a fluorescent dye and quenching fluorescence effect by chloramphenicol in continuous system. All the optimum parameters like fluorescent dye concentration, pH, flow rate, sample volume, and coil addition have been investigated. The CHP dynamic range was 0.1-3.0 mMol.L-1 with correlation coefficient (r) =0.9836 while the ( %r2) percentage linearity was %96.75. Limit of Detection is depended on the dilution of minimum concentration in calibration curve 1.680 g/Sample. The repeatability for 1 and 3 mMol.L-1 of CHP solution was found to be less than 1 % (n=6).The proposed method was successfully applied to determine the CHP in pharmaceutical formulations (capsules and eye drop).
Keywords: chloramphenicol, spectrofluorometric, 2, 7-dichlorofluorescein, laser diode 405 nm, flow injection analysis