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Research Paper | Biochemistry | Nigeria | Volume 6 Issue 7, July 2017
The Kinectics and Inhibition Properties of Partially Purified Glutathione Transferase from the Head of Palm Weevil (Rynchophorus Phoenicis) Larva
Oyewole O. Nather [3] | Falodun A. Emmanuel
Abstract: An insect cytosolic glutathione transferase (GST) was partially purified from the head of palm weevil (Rynchophorous phoenicis) larvae using DEAE- cellulose ion exchange chromatography. The purified GST showed conjugating activity readily with 1- chloro- 2, 4 dinitrobenzene (CDNB) and the activity was inhibited by an insecticide 2, 2-dichloroethenyl dimethyl phosphate (DDVP). Palm weevil GST displayed a Michealian behavior at low (0.1-1.0 mM) substrate concentration. Km (GSH), Vmax was calculated to be 0.70 mM and 40.98 mol/min/mg whereas Km and Vmax (CDNB) was 33.2 mol/min/mg and1.55 mM respectively. GST from the head of palm weevil larva probabily possesses ability to catalyse external toxic substances and may be implicated in GST-based resistance.
Keywords: Kinectics, Inhibition, glutathione transferase, Rynchophorus phoenicis
Edition: Volume 6 Issue 7, July 2017,
Pages: 918 - 921
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