Ailanthus excelsa and Balanites aegyptiaca are two wild growing common plant species of desert, having medicinal importance. Unorganized tissues of these useful plants were established on MS medium supplemented with 1.0mg/L BAP+1.5mg/L 2, 4-D and 1.5mg/L BAP +2.0mg/L 2, 4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations (1, 2, 3 mg/L) of growth regulators ( IAA, NAA ) and ( 10, 20, 30mg/L ) salts ( NaCl, KCl ) separately. Tissues at the maximum GI (in all samples) were harvested, dried, powdered and analyzed for estimation of ascorbic acid. Maximum amount of ascorbic acid was calculated in callus fed with 1mg/L IAA, NAA and 20 mg/L NaCl and KCl both in Ailanthus excelsa and Balanites aegyptiaca.