Research Paper | Microbiology | India | Volume 3 Issue 6, June 2014
Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste
Manish Kumar, Sushma
Phytase; an acid phosphatase enzyme; efficiently cleaves phosphate moieties from phytic acid thereby generating myo-inositol derivatives and inorganic phosphate; was indentified during screening of endophytic fungi Aspergillus niger. Phytase was produced in selective media at room temperature for 3 days. Production media was also optimized varying the concentration of ingredients from 5 to 25g by using solid-state fermentation for 7 days to check the activity of phytase. The zone of inhibition of crude and pure phytase were 2.1 and 2.4cm recorded using well diffusion method.
Keywords: phytase, wheat bran waste, inorganic phosphate, Aspergillus niger, solid state fermentation
Edition: Volume 3 Issue 6, June 2014
Pages: 949 - 951
How to Cite this Article?
Manish Kumar, Sushma, "Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=2014204, Volume 3 Issue 6, June 2014, 949 - 951