Downloads: 112 | Views: 120
Research Paper | Microbiology | India | Volume 4 Issue 10, October 2015
Production and Immobilization of extracellular Lipase from Pseudomonas aeruginosa.
Vivek Vishwe  | Abhay Chowdhary 
Abstract: Lipases (Triacylglycerol acylhydrolases, EC 184.108.40.206) catalyzes the enzymatic hydrolysis of triacylglycerol into diacylglycerol & free fatty acids. Lipases act as a key enzyme that is used in various industries like Detergent, Food, Leather, Textiles, Pharmaceuticals, etc. The present study investigates the potentials of Pseudomonas spp. isolated from oil contaminated soil samples in the production and immobilization of Lipase enzyme. Submerged fermentation of SP45 (Pseudomonas aeruginosa) was performed for the production of Lipase. Extracellular SP45 Lipase was then partially purified by ammonium sulphate precipitation followed by dialysis. SP45 Lipase precipitated with 50 % saturated solution of ammonium sulphate showed optimum Lipase activity of about 56.0433U/ml and it was increased upto 101.2400U/ml after dialysis. Purified SP45 Lipase was then immobilized using physical adsorption and microencapsulation methods. SP45 Lipase immobilized in sodium alginate beads showed greater enzymatic activity when compared with SP45 Lipase immobilized with ion exchange resins.
Keywords: Lipase, Pseudomonas aeruginosa, Fermentation, Immobilization, Spectrophotometric assay
Edition: Volume 4 Issue 10, October 2015,
Pages: 1479 - 1482
Similar Articles with Keyword 'Lipase'
Research Paper, Microbiology, India, Volume 10 Issue 10, October 2021Pages: 1486 - 1490
Characterization of Potential Enzyme - Producing Bacteria from Kitchen Waste
Downloads: 6 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2
Research Paper, Microbiology, India, Volume 11 Issue 12, December 2022Pages: 484 - 488
Isolation and Characterization of Hydrolytic Halophiles from Sea Water
Ruksana Beegam | Farsana Thasni C. P. | Fathima Rahana P. | Muhsina T. | Sahla K. P.