HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper | Environmental Science Studies | India | Volume 4 Issue 10, October 2015

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Estuarine grey mullet is very sensitive to several environmental factors. Apoptosis signal regulating kinase-1 (ASK1) activity is regulated at multiple levels, one of which is through inhibition by heat shock protein (HSP70). In this study, we investigated the inhibitory effect of pifithrin- in the hepatocytes of Mugil cephalus inhabiting Kovalam and Ennore estuaries. The level of total antioxidant capacity (TAC) and the expression of 4-hydroxy-2-nonenal (HNE), HSP70, HO-1, phospho-ERK and ASK1 were measured in hepatocytes of M. cephalus inhabiting Kovalam and Ennore estuaries with and without pifithrin- incubation. A significant increase in oxidative stress along with decrease in antioxidant status in hepatocytes of M. cephalus inhabiting Ennore estuary was observed, whereas pifithrin- incubation moderately alters the stress and antioxidant status. The level of HSP70 was inhibited by 39 % at 10 M of pifithrin- for 60 minutes. Pifithrin- enhances the generation of ROS, possibly by inhibiting the anti-oxidant actions of HSP70. An alteration in the expression of HSP70 by pifithrin- inhibitor causes downregulation of phospho-ERK and upregulation of ASK1 expression. These proapoptotic changes in the stressed fish hepatocytes indicate that HSP70 threshold is a key factor in fish survival under polluted environmental stress.

Keywords: ASK1, ERK, Estuary, Inhibitor, Mugil cephalus, Survival

Edition: Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

Share this Article

How to Cite this Article?

Padmini Ekambaram, Tharani Jayachandran, "HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB158881, Volume 4 Issue 10, October 2015, 1143 - 1152

Enter Your Registered Email Address





Similar Articles with Keyword 'ERK'

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Share this Article

Downloads: 114 | Weekly Hits: ⮙6 | Monthly Hits: ⮙12

Research Paper, Environmental Science Studies, India, Volume 5 Issue 5, May 2016

Pages: 2452 - 2455

Determination of Heavy Metals in Industrial Waste Waters of Tirupati Region, Andhra Pradesh

Putaka Ramesh, T. Damodhram

Share this Article

Downloads: 118 | Weekly Hits: ⮙4

Research Paper, Environmental Science Studies, India, Volume 5 Issue 9, September 2016

Pages: 1698 - 1701

Removal of Copper from Industrial Wastewater by Ion Flotation with Sodium Dodecyl Sulfate as Anionic Collector

Fouad S. M. Rashed, Satyendra Nath

Share this Article

Downloads: 130 | Weekly Hits: ⮙4 | Monthly Hits: ⮙17

Research Paper, Environmental Science Studies, India, Volume 3 Issue 9, September 2014

Pages: 316 - 320

Bioaugmentation for Dairy Wastewater

Amanpreet Kaur, Sonia Chaman

Share this Article

Downloads: 207 | Weekly Hits: ⮙3 | Monthly Hits: ⮙14

Research Paper, Environmental Science Studies, Nigeria, Volume 8 Issue 12, December 2019

Pages: 10 - 17

Risk Assessment of Heavy Metals in Soil and Vegetables of Selected Dumpsites in Port-Harcourt Metropolis

Onwugbuta N, Anaero-Nweke G. N., Ekweozor I. K. E., Ubong I. U

Share this Article

Similar Articles with Keyword 'Estuary'

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Share this Article

Downloads: 112 | Weekly Hits: ⮙1

Research Paper, Environmental Science Studies, Nigeria, Volume 9 Issue 4, April 2020

Pages: 1352 - 1362

Assessment of: Benzene, Toluene Ethylbenzene, Xylene in Water and Sediments from Itu, Ibeno and Ibaka River Estuary of Akwa Ibom State, Nigeria

Kalu O. Obasi, Iwok Enimeno Sunday, A.C. Udebuani, J.N.Okereke

Share this Article

Downloads: 114 | Weekly Hits: ⮙3 | Monthly Hits: ⮙9

Research Paper, Environmental Science Studies, India, Volume 4 Issue 3, March 2015

Pages: 2332 - 2337

Assessment of Heavy Metals Contamination Level in Estuarine Sea Sediment from Kollidam, India

K. Shameem Rani

Share this Article

Downloads: 129 | Weekly Hits: ⮙2

Research Paper, Environmental Science Studies, Nigeria, Volume 8 Issue 1, January 2019

Pages: 2148 - 2159

Eagle Island Wetland Mapping Using Satellite Imagery and Bathymery

Amina S. Dienye, Dagogo M. J Fubara

Share this Article

Downloads: 143 | Weekly Hits: ⮙4 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, Cameroon, Volume 5 Issue 8, August 2016

Pages: 264 - 271

A Trophic Structure Model of the Douala-Edea Reserve Mangrove (Cameroon) with Consideration of Sustainable Utilization of its Resources

Longonje Simon, Findi Emilien

Share this Article

Similar Articles with Keyword 'Inhibitor'

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Share this Article

Downloads: 113 | Monthly Hits: ⮙4

Review Papers, Environmental Science Studies, India, Volume 6 Issue 5, May 2017

Pages: 2353 - 2363

Metal Ion Inhibitors and Other Inorganic Toxicants in Anaerobic Biological Treatment Process of Industrial Effluents: A Critical Review

Dr. Mishu Singh

Share this Article

Similar Articles with Keyword 'Mugil cephalus'

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Share this Article

Downloads: 117 | Weekly Hits: ⮙2 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 6 Issue 7, July 2017

Pages: 1046 - 1050

The Effect of Water pH on Ventilation Frequency and Oxygen Consumption in Mullet (Mugil cephalus)

M. K. Kanagaraj, Lakshmipathi M. T, Asta Zerue, Samuel Berhane, Yemane Mobaetsion, T.J. Ramesha, A. T. Ramachandra Naik

Share this Article

Downloads: 163 | Weekly Hits: ⮙4 | Monthly Hits: ⮙10

Research Paper, Environmental Science Studies, India, Volume 7 Issue 7, July 2018

Pages: 731 - 738

The Bioaccumulation of Heavy Metals in Kollidam Estuary Edible Fish Mugil cephalus

Shameem Rani.K

Share this Article

Similar Articles with Keyword 'Survival'

Downloads: 111 | Weekly Hits: ⮙1 | Monthly Hits: ⮙13

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1143 - 1152

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes

Padmini Ekambaram, Tharani Jayachandran

Share this Article

Downloads: 112 | Weekly Hits: ⮙1 | Monthly Hits: ⮙11

Case Studies, Environmental Science Studies, Nigeria, Volume 4 Issue 4, April 2015

Pages: 2104 - 2108

The Impact of Soil Temperature on Miscanthus (Elephant) Plant: A Case study of Compton Experimental Site, West Midlands, UK

Aminu Mansur

Share this Article

Downloads: 115

Research Paper, Environmental Science Studies, India, Volume 4 Issue 10, October 2015

Pages: 1041 - 1042

Potentiality of Gobar Gas Generation and Population: A Case Study of Shrirampur Tahsil, Dist- Ahmednagar, M.S

Shete Shankar S.

Share this Article

Downloads: 117 | Weekly Hits: ⮙3 | Monthly Hits: ⮙12

Research Paper, Environmental Science Studies, India, Volume 8 Issue 12, December 2019

Pages: 1150 - 1153

Coir Pith as a Acclimatizing Medium for Micropropagated Capsicum SP

Immanuel Suresh J, Samuel Paul Raj

Share this Article

Downloads: 119 | Weekly Hits: ⮙3 | Monthly Hits: ⮙15

Research Paper, Environmental Science Studies, Nigeria, Volume 4 Issue 10, October 2015

Pages: 1910 - 1916

Assessment of the Bioaccumulation and the Excretion Rate of Cd, Zn and Pb in Blood, Kidney and Liver of an African Catfish Juvenile in an Artificial Fish Pond

Irerhievwie G. O., Akpoghelie J. O.

Share this Article



Top