Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064

Downloads: 107

Research Paper | Biology | United States of America | Volume 4 Issue 9, September 2015

Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death

Veronica L. Stallings, Kashenya M. Gurley, Checo J. Rorie

Triple-negative breast cancer (TNBC) is a complex disease that lacks the expression of the estrogen, progesterone, and HER2 receptors. Over 80 % of TNBC cells have a mutated p53 tumor suppressor gene. The p53 pathway is responsible for a cells arrest or death response to stress. Here we show that the TNBC cell lines HCC1806 and HCC70 expresses the p53 protein and its downstream targets p21 and Puma. This study also revealed that the native forms of the p53 protein were not detected by immunofluorescence assays. Here we analyzed the cell death responses in triple-negative breast cancer cell lines HCC70 and HCC1806. We exposed the TNBC cell lines HCC70 and HCC1806, and a normal breast cell line AG11132 to the chemotherapeutics staurosporine, Actinomycin D, and doxorubicin for 24 hrss. We observed the physical characteristics of apoptosis in both HCC70 and HCC1806. We also performed Western blot analysis to reveal that Parp was cleaved in the TNBC cell lines. In conclusion, cell death responses and apoptosis occurred in the TNBC cell lines HCC70 and HCC1806 even though they have mutated p53 genes. Therefore, more experiments need to be conducted to determine whether apoptosis occurs in a p53-dependent or independent manner.

Keywords: triple negative breast cancer, TNBC, p53, apoptosis

Edition: Volume 4 Issue 9, September 2015

Pages: 1024 - 1028

Share this Article

How to Cite this Article?

Veronica L. Stallings, Kashenya M. Gurley, Checo J. Rorie, "Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB158256, Volume 4 Issue 9, September 2015, 1024 - 1028

Enter Your Email Address




Similar Articles with Keyword 'triple negative breast cancer'

Downloads: 107

Research Paper, Biology, United States of America, Volume 4 Issue 9, September 2015

Pages: 1024 - 1028

Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death

Veronica L. Stallings, Kashenya M. Gurley, Checo J. Rorie

Share this Article

Downloads: 128

Research Paper, Biology, United States of America, Volume 4 Issue 9, September 2015

Pages: 73 - 78

Cancer Associated MicroRNAs are Differentially Expressed in Triple Negative Breast Cancer and Normal Breast Cells

Sherette S. Godfrey, Malcolm M. Moses, Checo J. Rorie

Share this Article

Similar Articles with Keyword 'TNBC'

Downloads: 107

Research Paper, Biology, United States of America, Volume 4 Issue 9, September 2015

Pages: 1024 - 1028

Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death

Veronica L. Stallings, Kashenya M. Gurley, Checo J. Rorie

Share this Article

Downloads: 128

Research Paper, Biology, United States of America, Volume 4 Issue 9, September 2015

Pages: 73 - 78

Cancer Associated MicroRNAs are Differentially Expressed in Triple Negative Breast Cancer and Normal Breast Cells

Sherette S. Godfrey, Malcolm M. Moses, Checo J. Rorie

Share this Article

Similar Articles with Keyword 'apoptosis'

Downloads: 67 | Monthly Hits: ⮙1

Research Paper, Biology, India, Volume 9 Issue 12, December 2020

Pages: 438 - 448

Identification and Analysis of GeneExpression in Flies Brain: An Insilico Approach

Arpita Parakh, Naveen Dhingra

Share this Article

Downloads: 107

Research Paper, Biology, United States of America, Volume 4 Issue 9, September 2015

Pages: 1024 - 1028

Triple Negative Breast Cancer Cell Lines with TP53 Mutations are Able to Undergo Cell Death

Veronica L. Stallings, Kashenya M. Gurley, Checo J. Rorie

Share this Article

Downloads: 113

Research Paper, Biology, India, Volume 4 Issue 4, April 2015

Pages: 1950 - 1954

An Integrative Approach for In Silico Study on Treating Molluscum Contagiosum with Snail Slime

Deepak Tom Jose, Caroline Nirmala. R

Share this Article

Downloads: 114

Research Paper, Biology, India, Volume 3 Issue 6, June 2014

Pages: 1111 - 1120

Comparative Evaluation of Immune Protective Effect of Melatonin and Boerhaavia diffusa in Lymphoid Tissues of Park's Strain Mice against Antibiotic Induced Oxidative Stress

Somenath Ghosh, Shweta Sharma, Abhinav Joshi, Chandana Haldar

Share this Article

Downloads: 143

Research Paper, Biology, India, Volume 2 Issue 9, September 2013

Pages: 12 - 18

Caspase Dependent Apoptosis is Only Inhibited on y Irradiation of Cells Conditioned by Repetitive Oxidative Stress

Rita Ghosh, Koyeli Girigoswami, Dipanjan Guha

Share this Article



Top