Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Biology | India | Volume 4 Issue 8, August 2015

Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade

Tomato (Solanum lycopersicum L.) and Chilli (Capsicum annuum L.) are the most important and widely grown vegetable crop in the world belonging to the family Solanaceae. Tomato is important source of vitamins A and C and Chillies are excellent source of Vitamin A, B, C and E. India is one of the major producers of chilli and tomato. Leaf curl is one of the most important diseases of chilli and tomato causes substantial losses every year. The Tomato Leaf Curl Virus Disease (ToLCVD) is characterized by the upward and downward curling and twisting of leaves followed by marked reduction in leaf size. The diseased plants looks pale and stunted due to shortening of inter nodal length with more lateral branches resulting in a bushy appearance of leaves and shortening of internodes with partial to complete sterility. The DNA extracted by CTAB method from the leaf sample. For identification of ToLCV disease of tomato and chilli primers were designed using NCBI tool by collecting sequences of ToLCV from different regions of India. DNA sample was amplified successively with designed primers using PCR cycles and the band appearance was observed on the gel electrophoresis. From the appearance of the band the disease identification and diagnosis of Tomato leaf curl virus ToLCV was done. In future work ToLCV disease could be confirmed using NESTED PCR.

Keywords: Chilli, NCBI, NESTED PCR, ToLCV, Tomato

Edition: Volume 4 Issue 8, August 2015

Pages: 1713 - 1716

Share this Article

How to Cite this Article?

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade, "Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB157720, Volume 4 Issue 8, August 2015, 1713 - 1716

42 PDF Views | 41 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'Chilli'

Research Paper, Biology, India, Volume 4 Issue 10, October 2015

Pages: 1787 - 1790

Development and Sensory Attributes of the Pickle made from Bitter Gourd and Bottle Gourd

Sangeeta A. Pingale, Vaibhav R. Dabhade

Share this Article

Research Paper, Biology, India, Volume 4 Issue 8, August 2015

Pages: 1713 - 1716

Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade

Share this Article

Research Paper, Biology, India, Volume 5 Issue 4, April 2016

Pages: 727 - 729

Efficay of Plant Extracts and Vermiwash on Dynamics of Thrips Population and Yield of Chillies

S. Sendur Kumaran

Share this Article

Similar Articles with Keyword 'NCBI'

Research Paper, Biology, India, Volume 3 Issue 6, June 2014

Pages: 564 - 571

Human ATP Binding Cassette (ABC) Transporters: A Phylogenetic Investigation

Sandeep Kumar Vishwakarma, Syed Ameer Basha Paspala, Aleem Ahmed Khan

Share this Article

Research Paper, Biology, India, Volume 3 Issue 6, June 2014

Pages: 1792 - 1800

Functional Characterization and Structure Prediction of HDAC-10: A Bioinformatics Approach

Sabeena M, Kaiser Jamil

Share this Article

Research Paper, Biology, India, Volume 4 Issue 8, August 2015

Pages: 1713 - 1716

Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade

Share this Article

M.Tech / M.E / PhD Thesis, Biology, India, Volume 3 Issue 6, June 2014

Pages: 1398 - 1403

Isolation; Screening and Purification of Cellulase from Cellulase Producing Klebsiella variicola RBEB3 (KF036184.1)

Gopinath.S.M, Ismail Shareef, Ashalatha, Shreya Ranjit

Share this Article

Research Paper, Biology, India, Volume 3 Issue 11, November 2014

Pages: 2045 - 2051

Evaluation of Castor Ecotypes of Selected Regions of the Western Ghats of Karnataka, India through Bio-Chemical Assay

K. G. Manjunath, B. Sannappa

Share this Article

Similar Articles with Keyword 'NESTED PCR'

Research Paper, Biology, India, Volume 4 Issue 8, August 2015

Pages: 1713 - 1716

Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade

Share this Article

Research Paper, Biology, Saudi Arabia, Volume 6 Issue 3, March 2017

Pages: 93 - 98

Morphological and Molecular Identification of Malaria Vector in Three Sites in Kordofan Region with Exposure of Plasmodium Falciparum as Malaria Parasite

Fathielhag, Sumaiaabukashawa, Hind abushama

Share this Article

Similar Articles with Keyword 'Tomato'

Research Paper, Biology, Macedonia, Volume 3 Issue 9, September 2014

Pages: 2140 - 2141

Identifying of Allergic Agents to Different Human Age Groups in Tetovo Area

Enver Zenku, Sheval Memishi, Agim Haziri

Share this Article

Research Paper, Biology, Sudan, Volume 3 Issue 7, July 2014

Pages: 1324 - 1328

The Antifungal Effects of Four Tomato Rhizosphere Bacillus spp. against Alternaria Alternata

Azza Siddig Abbo, Mohamed Osman Idris, Ahmed Mohamed Hammad

Share this Article

Research Paper, Biology, India, Volume 4 Issue 5, May 2015

Pages: 2996 - 2998

Phylogenetic Analysis of Dietary Flavonoids Against COX-2 Protein Causing Colon Cancer

Meena Sharma, Shallu Kalia

Share this Article

Research Paper, Biology, India, Volume 4 Issue 10, October 2015

Pages: 1775 - 1779

Development of Tomato Sauce Fortified with Bael and its Sensory and Chemical Evaluation

Pingale Sangeeta Avinash, Dighe Nilesh Madhav

Share this Article

Research Paper, Biology, India, Volume 4 Issue 8, August 2015

Pages: 1713 - 1716

Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli

A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade

Share this Article
Top