Research Paper | Biology Science | Kenya | Volume 4 Issue 8, August 2015
Distribution of Colonization Factors and Toxins among Diarrheal Children below 5Years: A Case Study of Moi Teaching and Referral Hospital, Kenya
Faith Jeruto, Dr. Lizzy Mwamburi, Dr. James Wanga
Estimates suggest that approximately 200 million cases and 380, 000 deaths occur in children below 5 years of age due to Enterotoxigenic Escherichia coli (ETEC) infections annually. The study aimed at molecular determination of ETEC toxins and colonization factors distribution among children below 5 years of age. Multiplex PCR was used in the identification of ETEC toxin and colonization factors. A total of 200 isolates of E.coli-like recovered from diarrheic children at Moi Teaching and Referral Hospital were collected and transported to Microbiology Hub Walter Reed Project-Kericho and tested for the presence of virulent factors using Multiplex PCR method. Positive isolates for ETEC were subjected to a panel of colonization factor antigens (CFA) using multiplex PCR for identification of colonization factors. Data was analyzed using statistical analysis system (SAS) and fishers exact test was used for comparing 2 variables. Heat labile toxins (LTh) were more common (65.38 %) than heat stable toxins (STh) (26.92 %). LTh ETEC toxins were observed in younger and older children (42.3 % and 23.08 % respectively) while STh ETEC toxins were more frequent in children below 2 years and were not observed in children above 2 years old. The most common colonization factor was coli surface antigens 6 (Cs6) and coli surface antigens 1 (Cs1) and was detected in children below 2 years of age. STh and LTh ETEC toxins had a detectable Colonization factor at higher frequency (19.23 %). Children below 5years are susceptible to ETEC toxins and CFs. Use of molecular diagnostic technique to determine the distsribution of ETEC toxins and CFs should be adopted.
Keywords: Enterotoxigenic Escherichia coli, ETEC toxins, colonization factors
Edition: Volume 4 Issue 8, August 2015
Pages: 1161 - 1164
How to Cite this Article?
Faith Jeruto, Dr. Lizzy Mwamburi, Dr. James Wanga, "Distribution of Colonization Factors and Toxins among Diarrheal Children below 5Years: A Case Study of Moi Teaching and Referral Hospital, Kenya", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB157152, Volume 4 Issue 8, August 2015, 1161 - 1164
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