Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Biology | Pakistan | Volume 4 Issue 6, June 2015

Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source

Ikram-ul Haq, Zahra Arshad, Taseer

The effect of commercial fruit juices was assessed on callus induction, its proliferation and plant regeneration in cucumber (Cucumis sativus L.) cv., Liza. Almost 3-4 mm, cuttings of seedling (7-10 days old) were cultured for callus induction on MS2 [MS0 (Murashige & Skoog, 1962) basal salts, 0.6 mg L-1 2, 4-D (2, 4-Dichlorophenoxy acetic acid), 0.4 mg L-1 BAP (Benzyleaminopurine) ] medium and also on simple MS0 medium supplemented with fruit juices in place of 3 % sucrose like as MS2a (MS0 with orange juice), MS2b (apple), MS2c (red grapes), MS2d (strawberry) for callus induction. No callusing was observed on fruits juice cultures while callus proliferation was higher in orange juice culture (MS2a), when 3-weeks old callus sub-cultured from MS2 medium. Maximum plant regeneration (3.980.65 per callus) was observed on *MS2c medium when somatic embryogenesis was induced on MS3 (0.1 mg L-1 BAP, 0.5 mg L-1 glutamate) medium in dark (2-weeks). Root induction efficiency was higher in 1MS2b and 1MS2d 1/2MS) cultures under light condition. After plant hardening, they were transferred to soil conditions.

Keywords: Cucumis sativus L, callus induction, fruit juices, somatic embryogenesis, plant regeneration

Edition: Volume 4 Issue 6, June 2015

Pages: 81 - 84

Share this Article

How to Cite this Article?

Ikram-ul Haq, Zahra Arshad, Taseer, "Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB154295, Volume 4 Issue 6, June 2015, 81 - 84

55 PDF Views | 53 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'callus induction'

Research Paper, Biology, Sudan, Volume 3 Issue 7, July 2014

Pages: 1698 - 1702

A Simple and Efficient Protocol for Callus Induction and Regeneration from Wheat (Triticum aestivum L.) Mature Embryos

Badr Eldin A. E. Saeed, Marmar A. El Siddig, Aisha Ohag Osman, Adil A. El Hussein

Share this Article

Research Paper, Biology, Pakistan, Volume 4 Issue 6, June 2015

Pages: 81 - 84

Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source

Ikram-ul Haq, Zahra Arshad, Taseer

Share this Article

Research Paper, Biology, Bangladesh, Volume 2 Issue 8, August 2013

Pages: 162 - 164

Micropropagation of Hoya Kerrii (Valentine Hoya) Through Callus Induction for Long Term Conservation and Dissemination

Romana Siddique

Share this Article

Similar Articles with Keyword 'somatic embryogenesis'

Research Paper, Biology, Saudi Arabia, Volume 5 Issue 6, June 2016

Pages: 2263 - 2268

Gibberellic Acid and Indole 3 Butyric Acid Regulation of Maturation and Accumulation of Storage Proteins (56, 34 and 26 KD) in Somatic Embryos of Santalum album L.

Abdul Ilah, Mazahirul Islam Syed, A. M. Reyad, Abdul Mujib

Share this Article

Research Paper, Biology, India, Volume 4 Issue 11, November 2015

Pages: 2404 - 2408

Regeneration of Plantlets via Direct Somatic Embryogenesis from Different Explants of Murraya koenigii. (L.) Spreng

Nisha Khatik, Ramesh Joshi

Share this Article

Research Paper, Biology, Pakistan, Volume 4 Issue 6, June 2015

Pages: 81 - 84

Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source

Ikram-ul Haq, Zahra Arshad, Taseer

Share this Article

Similar Articles with Keyword 'plant regeneration'

Research Paper, Biology, India, Volume 4 Issue 11, November 2015

Pages: 2404 - 2408

Regeneration of Plantlets via Direct Somatic Embryogenesis from Different Explants of Murraya koenigii. (L.) Spreng

Nisha Khatik, Ramesh Joshi

Share this Article

Research Paper, Biology, Pakistan, Volume 4 Issue 6, June 2015

Pages: 81 - 84

Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source

Ikram-ul Haq, Zahra Arshad, Taseer

Share this Article
Top